Preparation of Chitosan-Based Hemostatic Sponges by Supercritical Fluid Technology

نویسندگان

  • Hu-Fan Song
  • Ai-Zheng Chen
  • Shi-Bin Wang
  • Yong-Qiang Kang
  • Shi-Fu Ye
  • Yuan-Gang Liu
  • Wen-Guo Wu
چکیده

Using ammonium bicarbonate (AB) particles as a porogen, chitosan (CS)-based hemostatic porous sponges were prepared in supercritical carbon dioxide due to its low viscosity, small surface tension, and good compatibility with organic solvent. Fourier transform infrared spectroscopy (FTIR) spectra demonstrated that the chemical compositions of CS and poly-(methyl vinyl ether-co-maleic anhydride) (PVM/MA) were not altered during the phase inversion process. The morphology and structure of the sponge after the supercritical fluid (SCF) process were observed by scanning electron microscopy (SEM). The resulting hemostatic sponges showed a relatively high porosity (about 80%) with a controllable pore size ranging from 0.1 to 200 µm. The concentration of PVM/MA had no significant influence on the porosity of the sponges. Comparative experiments on biological assessment and hemostatic effect between the resulting sponges and Avitene® were also carried out. With the incorporation of PVM/MA into the CS-based sponges, the water absorption rate of the sponges increased significantly, and the CS-PVM/MA sponges showed a similar water absorption rate (about 90%) to that of Avitene®. The results of the whole blood clotting experiment and animal experiment also demonstrated that the clotting ability of the CS-PVM/MA sponges was similar to that of Avitene®. All these results elementarily verified that the sponges prepared in this study were suitable for hemostasis and demonstrated the feasibility of using SCF-assisted phase inversion technology to produce hemostatic porous sponges.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Application of Supercritical Fluid ‎Technology for Preparation of Drug Loaded ‎Solid Lipid Nanoparticles

   Small changes in pressure or temperature, close to the critical point, lead to large changes in solubility of supercritical carbon dioxide (CO2). Environmentally friendly supercritical CO2 is the most popular and inexpensive solvent which has been used for preparation of nanodrugs and nanocarriers in drug delivery system with supercritical fluid technology. Delivery...

متن کامل

Effect of a chitosan-based hemostatic dressing on blood loss and survival in a model of severe venous hemorrhage and hepatic injury in swine.

BACKGROUND Hemorrhage is a leading cause of death from trauma. An advanced hemostatic dressing could augment available hemostatic methods. We studied the effects of a new chitosan dressing on blood loss, survival, and fluid use after severe hepatic injury in swine. METHODS Swine received chitosan dressings or gauze sponges. Standardized, severe liver injuries were induced. After 30 seconds, d...

متن کامل

Hydrothermal synthesis nanoparticle bysupercritical fluid

Supercritical fluid (SCF) technology has become an important tool of materials processing in the lasttwo decades. Supercritical CO2 and H2O are extensively being used in the preparation of a great variety ofnanomaterials. The greatest requirement in the application of nanomaterials is its size and morphology control, which determine the application potential of the nanoparticles, as...

متن کامل

Determination of efficacy of novel modified chitosan sponge dressing in a lethal arterial injury model in swine.

BACKGROUND Chitosan is a functional biopolymer that has been widely used as a hemostat. Recently, its efficacy has been questioned due to clinical failures as a result of poor adhesiveness. The purpose of this study was to compare, in a severe groin injury model in swine, the hemostatic properties of an unmodified standard chitosan sponge with standard gauze dressing and a novel hydrophobically...

متن کامل

Determination of efficacy of a novel alginate dressing in a lethal arterial injury model in swine.

INTRODUCTION Alginate is a biocompatible polysaccharide that is commonly used in the pharmaceutical, biomedical, cosmetic, and food industries. Though solid dressings composed of alginate can absorb water and promote wound healing, they are not effective hemostatic materials, particularly against massive hemorrhage. The purpose of this study is to attempt to increase the hemostatic capabilities...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2014